))
Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches
Manage episode 435036055 series 3391756
Konten disediakan oleh Bitesize Bio. Semua konten podcast termasuk episode, grafik, dan deskripsi podcast diunggah dan disediakan langsung oleh Bitesize Bio atau mitra platform podcast mereka. Jika Anda yakin seseorang menggunakan karya berhak cipta Anda tanpa izin, Anda dapat mengikuti proses yang diuraikan di sini https://id.player.fm/legal.
Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions.
Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions.
Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence.
Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time.
Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com
124 episode
Bagikan
